Nipt blood test results
Non-invasive prenatal testing (NIPT) | Pregnancy Birth and Baby
Non-invasive prenatal testing (NIPT) | Pregnancy Birth and Baby beginning of content6-minute read
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Key facts
- The non-invasive prenatal test (NIPT) screens your baby for genetic health conditions.
- You may want to consider genetic counselling before having an NIPT to help you make an informed decision.
- If your NIPT result shows that your baby is likely to have a chromosomal difference, a diagnostic test such as chorionic villus sampling or amniocentesis can confirm your result.
What is the non-invasive prenatal test (NIPT)?
The non-invasive prenatal test (NIPT) is a very accurate screening test. Screening tests are used to see if your baby has a high chance of a genetic health condition. These conditions include Down syndrome and other chromosomal differences. The NIPT involves a simple blood test that is done in your first trimester of pregnancy.
There are different kinds of prenatal testing available to check the health of your baby. It’s your choice if you would like to have these tests. Talk about your options with your doctor or genetic counsellor and give yourself some time to make your decision.
Screening tests are different to diagnostic tests. Diagnostic tests are often more invasive. However they confirm for certain if the result is positive.
During pregnancy, some of the baby’s DNA passes into your bloodstream. The non-invasive prenatal test analyses the genetic information contained in this DNA. It’s used to screen for a number of genetic conditions. The test is particularly sensitive to Down syndrome. It was first offered in Australia in 2012.
In Australia, NIPTs are offered in private centres and involve an out-of-pocket cost. They are sometimes referred to by different names, depending on the company that makes them. They might be called: Harmony, Generation or Percept.
An NIPT is done from 10 weeks into the pregnancy. Before the test you will be asked to give consent.
What does it test for?
The NIPT is a safe and very effective way of screening for certain conditions. These include:
- Down syndrome (also called trisomy 21)
- Edwards syndrome (trisomy 18)
- Patau syndrome (trisomy 13)
- Turner syndrome
Some laboratories also test the sex of your baby and look for differences with the sex chromosomes.
The test identifies many chromosomal anomalies. It doesn’t screen for genetic disorders such as:
- cystic fibrosis
- thalassaemia
- sickle cell anaemia
Screening will tell you how likely it is that your baby has a chromosomal difference. The only way of knowing for sure is to have a diagnostic test such as:
- chorionic villus sampling (CVS)
- amniocentesis
Should I have an NIPT?
The NIPT is very sensitive. It picks up more than 99% of cases of Down syndrome. But it is a screening test rather than a diagnostic test.
It can tell you whether there is an increased chance of having a baby with a genetic condition. It doesn’t give you a definitive answer. For some parents, information from screening tests can help them decide about whether to have diagnostic testing.
You might choose to have an NIPT test if:
- Your first trimester combined screening test shows you have an increased chance of having a baby with Down syndrome (this test combines results from a blood test at 10 to 12 weeks and an ultrasound at 11 to 13 weeks).
- You did not have the first trimester combined screening test because it was too late or the test wasn't available in your area.
- You want to understand your chance of having a baby with Down syndrome before considering diagnostic tests such as amniocentesis or CVS.
- You have an increased chance of having a baby with Down syndrome because you are older or you or your partner already have a baby with Down syndrome or another genetic condition.
It’s a good idea to consider genetic counselling before you have an NIPT to help you make an informed decision. It’s important to understand the risks and benefits of having the test.
Genetic counsellors can:
- review your family and medical history
- give you information about genetic tests
- help you learn about how the condition is inherited
- offer advice on support services
If your pregnancy is affected by a genetic condition, your genetic counsellor can:
- help support you
- help you understand what the results mean for you and your family
What can I expect from my NIPT results?
It can take up to 2 weeks to get the result of your NIPT.
If your NIPT result shows that your baby is likely to have a chromosomal difference, a diagnostic test such as: CVS or amniocentesis can confirm the result.
You should discuss your options with your doctor, midwife or genetic counsellor.
How much does the NIPT cost?
The NIPT is not covered by Medicare or private health insurance in Australia. You can expect to pay about $400 to $500 for an NIPT.
You may also need to pay for: an appointment with your doctor to get a referral, as well as an ultrasound.
You should speak with your doctor or a genetic counsellor before getting an NIPT. You can find genetic counselling services near you using the healthdirect Service Finder here.
You can read more about genetic counselling here.
If you have any questions about the NIPT, you can call the Pregnancy Birth and Baby helpline.
Speak to a maternal child health nurse
Call Pregnancy, Birth and Baby to speak to a maternal child health nurse on 1800 882 436 or video call. Available 7am to midnight (AET), 7 days a week.
Sources:
The Royal Women's Hospital Victoria (Genetic testing in pregnancy), NSW Health (Genetic and genomic testing for intellectual disability and childhood syndromes), O&G Magazine (Developments in non-invasive prenatal testing: is bigger better?), Allied Health Professions Australia (Genetic Counselling)Learn more here about the development and quality assurance of healthdirect content.
Last reviewed: September 2022
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NIPT Test (Noninvasive Prenatal Testing): What To Expect
Overview
What is the NIPT test?
NIPT stands for noninvasive prenatal testing. It’s a screening test offered during pregnancy to see if the fetus is at risk for having a chromosomal disorder like Down syndrome (trisomy 21), trisomy 18 (Edwards syndrome) and trisomy 13 (Patau syndrome). The test can also determine the sex of the fetus. It’s done by taking a sample of your blood, which also contains fragments of DNA from the fetus. DNA makes up a person’s genes and chromosomes, and gives healthcare providers a glimpse into the fetus’s genetic makeup. The blood sample is sent to a lab and analyzed for specific congenital disorders. NIPT can’t screen for all chromosomal or genetic conditions.
The NIPT test is also called cell-free DNA (cfDNA) screening or noninvasive prenatal screening (NIPS). It’s important to remember that a screening test estimates the likelihood of the fetus having a particular condition. It doesn’t diagnose a condition. NIPT testing is optional. Your healthcare provider will provide information about prenatal genetic testing and help you make an informed choice about your options.
What does the NIPT test screen for?
NIPT doesn’t test for all chromosomal conditions or birth disorders. Most NIPT tests screen for:
- Down syndrome (trisomy 21).
- Trisomy 18.
- Trisomy 13.
- Disorders affecting sex chromosomes (X and Y).
An extra chromosome causes Down syndrome, trisomy 18 and trisomy 13. Screening for sex chromosomes can help predict the sex of the fetus and can also screen for differences in the usual number of sex chromosomes. The most common sex chromosome conditions are Turner syndrome, Klinefelter syndrome, Triple X syndrome and XYY syndrome. Not all NIPT panels evaluate for the same conditions. It’s important to talk to your healthcare provider about what your NIPT is screening for.
Why is noninvasive prenatal testing done?
Noninvasive prenatal testing helps determine the fetus’s chances of being born with certain chromosomal disorders. Healthcare providers may recommend it if you:
- Have a child with a chromosomal abnormality.
- Have had an ultrasound that shows that the fetus may have an abnormality.
- Have had an earlier screening test that suggests a potential problem.
The American College of Obstetricians and Gynecologists (ACOG) used only to recommend NIPT for pregnant people considered high risk. However, it’s now recommended that providers offer NIPT to all pregnant people, regardless of risk.
Based on the results of the NIPT test, your obstetrician may recommend diagnostic tests. Diagnostic tests give a definitive answer about whether the fetus has a specific condition.
When should the NIPT test be done in pregnancy?
NIPT testing can be done as early as 10 weeks of pregnancy through delivery. There’s typically not enough fetal DNA in a pregnant person’s blood before 10 weeks of pregnancy.
How accurate are NIPT tests?
The accuracy of the test varies by the condition that it’s checking for. Other factors — like being pregnant with multiples, being a surrogate or having obesity — can affect NIPT results.
NIPT is about 99% accurate in detecting Down syndrome. The test is slightly less accurate for detecting trisomy 18 and 13. Overall, NIPT tests produce fewer false positives than other prenatal screenings like the quad screen.
Does the NIPT test show the sex of the fetus?
Yes, NIPT can predict the sex of the fetus.
Test Details
Is it necessary to get the NIPT test during pregnancy?
No, it’s not necessary. It’s a personal choice, and it’s normal to have questions. Your healthcare provider will discuss all your prenatal screening options, including NIPT. Many factors may go into your decision to have NIPT and prenatal genetic testing in general. If you’re having a difficult time or wish to discuss the screenings in more detail, a genetic counselor can help you understand the prenatal testing options and what may be a good fit for you.
How do doctors perform a NIPT test?
During this test, your provider takes a sample of your blood to look for abnormalities in the fetus’s DNA. Your DNA is inside all of your cells. Your cells are constantly dividing and creating new cells. When cells break down, tiny DNA fragments are released into your bloodstream. You have a small amount of the fetus’s DNA circulating in your bloodstream during pregnancy. The NIPT looks at these fragments of fetal DNA in your blood — known as cell-free DNA or cfDNA.
Your provider obtains a blood sample through a vein in your arm. They send this sample to a lab to analyze it for specific conditions.
It’s important to note that it takes about 10 weeks for enough fetal DNA to circulate in your blood. That’s why the screening isn’t performed until 10 weeks into the pregnancy.
Are there any risks to the NIPT test?
NIPT tests are safe, and there’s no risk to the fetus. It requires drawing blood from the pregnant person only.
Results and Follow-Up
When should I get my test results?
Results from NIPT tests can sometimes take up to two weeks, although results are often available sooner. Your healthcare provider will receive your test results first, then share the results with you.
What do the results of the NIPT test mean?
NIPT is a screening test, which means that it won’t give a yes or no answer about whether or not a fetus has a condition. The result will show if there’s an increased or decreased risk for a fetus to have the condition being screened. Your test results may sometimes be hard to interpret, so ask your healthcare provider for help if you’re unsure.
Most labs give separate results for each condition they’re screening for. For example, you may get a positive or high-risk result for trisomy 13 but a negative or low-risk result for Down syndrome.
There’s also the possibility that no test results are given due to insufficient fetal DNA in your blood or difficulty identifying fetal DNA. In this case, you can repeat the NIPT test and hope for a result the second time. Your healthcare provider can best guide you in these situations.
If noninvasive prenatal testing indicates that the fetus is at risk for a chromosomal disorder, your provider may recommend diagnostic testing. These tests diagnose conditions and give a yes or no answer:
- Amniocentesis: Amniocentesis is a procedure in which a small amount of amniotic fluid is taken from your uterus. This test can be performed after 15 weeks of pregnancy.
- Chorionic villus sampling (CVS): During chorionic villus sampling test, cells are taken from the placenta. The cell sample is sent to a lab. This test can be performed between 10 and 13 weeks of pregnancy.
It’s important to discuss the results of your NIPT with your provider so you have all the information you need to determine the next steps.
Can NIPT testing be wrong for Down syndrome?
NIPT is a screening test and, therefore, isn’t perfect. It’s important that you talk to your healthcare provider about your results and options to get more information.
Additional Details
Is the NIPT test worth it?
Getting a noninvasive pregnancy screening or other prenatal genetic test is up to you. Your healthcare provider can answer any questions you have, but ultimately you have to decide how a genetic or chromosomal disorder affects you and your family based on your situation.
The following questions might be helpful to you as you make your decision:
- How will I feel about a positive screening result?
- Would I consider diagnostic tests like amniocentesis or CVS?
- Would I do something different knowing the fetus has a genetic condition or an increased risk for a genetic condition?
- Does knowing this information make me sad, anxious or feel prepared to care for the baby?
- Would knowing this information help my provider(s) take better care of the baby?
How much does the NIPT test cost?
NIPT testing costs vary. Most health insurances cover most (if not all) of the cost. Many cover at least a portion. Check with your insurance provider before testing to be sure. If you don’t have insurance or your insurance doesn’t cover NIPT tests, you can pay for the test.
Can NIPT be done at 14 weeks?
Yes, NIPT can be done anytime after 10 weeks of pregnancy.
What questions should I ask my doctor about the NIPT test?
Noninvasive pregnancy screening tests are a personal choice. You may have questions about what your results mean or if you should even have the NIPT test. Don’t be afraid to ask questions. Remember, only you and your family can decide what’s best for you.
Some common questions to ask your healthcare provider are:
- Would you get the NIPT test if you were me?
- If my screening test is positive, what are the next steps?
- Is a genetic counselor available to talk to me about my options?
- What are the chances of false positives?
A note from Cleveland Clinic
The NIPT test is a highly reliable prenatal screening tool that assesses the risk of chromosomal disorders in a fetus. This test can also provide information about the sex of the fetus. NIPT testing doesn’t diagnose conditions — it only suggests a fetus is more likely to have a particular condition. Diagnostic testing may be recommended after you get NIPT test results. Prenatal tests like NIPT are optional, and getting the test is entirely up to you. Talk to your healthcare provider or a genetic counselor about your concerns. Be sure to understand what the test screens for and what the results mean so you make an informed decision.
Terms of laboratory tests
The dates are developed without taking into account the day of blood sampling.
The days of the week indicated in the table directly reflect the days of the study (in order for the biological material to be examined on this particular day / days, it must be taken the day before).
The setting schedule is indicative and can be adjusted according to the specific epidemiological situation.
Test results at the AIDS Center reception desk are issued after 17.00. nine0006
| Study name | Research periods |
| HIV testing | |
| | 1 working day |
| Tests for viral hepatitis | |
| | 4 working days |
| | 1 business day |
| | 1 working day |
| | 1 business day |
| Research on UGI and AIDS-associated infections | |
| | 1 business day |
| | 1 business day |
| | 1 business day |
| Cytomegalovirus infection (IgM, IgG, IgG-Avidity) | 1 business day (may be extended up to 3 business days) material release - Wednesday |
| Herpes infection (IgM, IgG, IgG-Avidity) | 1 working day (may be extended up to 3 working days) Material delivery - Friday |
| ELISA test for thyroid hormones | |
| | 1 business day |
| | 1 working day |
| | 1 business day |
| General clinical studies | |
| | 1 business day |
| | 1 business day |
| | 1 business day |
| | 1 business day |
| PCR studies | |
| | up to 14 working days |
| | up to 14 working days |
| | up to 14 working days |
| | up to 14 working days |
| | up to 14 working days |
| | up to 14 business days |
| Research and diagnosis of tick and tick infections | |
| | 1 working day |
| | 1 working day | nine0029
| | 1 working day |
| | 1 business day |
| | 1 working day |
| Research on COVID-19 | |
| Coronavirus, throat and nose swab, PCR | 2 days from the date of collection. |
| Coronavirus, blood, IgM, IgG, ELISA | 2 days from the date of collection. Setting daily, around the clock |
* the table shows the terms of the most requested laboratory tests
Complete blood count decoding | Clinical blood test transcript
Complete blood count is one of the most popular basic laboratory tests. It is used both in the diagnosis of many diseases, and in monitoring their course and treatment results. What is a general blood test, decoding of the term - later in the article.
What is a blood test
The clinical blood test is based on the study of red blood cells, also known as:
- red blood cells;
- leukocytes;
- platelets.
It requires about 5 ml of blood, usually drawn from an anticoagulant vein into a special anticoagulant tube. The test is usually performed prophylactically, but doctors may also recommend it, for example, if anemia, dehydration, or infection is suspected. A clinical blood test is also deciphered by a specialist doctor.
Erythrocytes in a blood test
One of the main indicators of a blood test is the erythrocyte system. Tests can be divided into 3 main groups, namely the number of red blood cells, their volume and hemoglobin concentration. Each of them allows you to set several important parameters. Among them are such values as:
-
- Hb (HBL, HGB) is the concentration of hemoglobin in the blood, measured in g/dl. HGB standards in the CBC have been found to be in the range of 13–18 g/dl for men and 12–16 g/dl for women and may vary slightly by laboratory. The exception is pregnant women with a normal blood count for this indicator of 11-14 g / dl
A low hemoglobin level is one of the most important criteria for determining anemia. A very common cause of elevated hemoglobin levels is dehydration and chronic hypoxia. The latter can be caused by lung disease, obstructive sleep apnea or congenital heart disease, and sometimes exposure to high altitude or smoking.
Other causes of elevated hemoglobin include polycythemia vera, glucocorticosteroid treatment, and some types of cancer. nine0003
Low hemoglobin in the blood test, in turn, this can be caused by chronic diseases, including autoimmune diseases, infections, kidney disease or cancer, as well as deficiency of vitamin B12, folic acid or iron, sudden loss of large amounts of blood and overload of the body. Sometimes a decrease in this indicator is associated with too rapid breakdown of red blood cells, congenital or resulting from bone marrow damage, which can occur, for example, with certain therapies or when taking certain drugs. nine0003
- MCV — mean erythrocyte volume. The correct value of this parameter in the general clinical blood test is 82-92 fl. If it is lower, then this may be due to iron deficiency, thalassemia, as well as hypertonic dehydration or overhydration, i.e. associated with an excess or lack of water itself, with a small proportion of electrolytes.
- Hb (HBL, HGB) is the concentration of hemoglobin in the blood, measured in g/dl. HGB standards in the CBC have been found to be in the range of 13–18 g/dl for men and 12–16 g/dl for women and may vary slightly by laboratory. The exception is pregnant women with a normal blood count for this indicator of 11-14 g / dl
Vitamin B12 and folic acid deficiencies, hematologic myelodysplastic syndromes, hypothyroidism, dehydration and hypotonic fluid overload, and diseases of the small intestine, liver, and stomach may cause an increase in MCV. Sometimes this blood count is also caused by alcoholism or pregnancy,
- MCH is the average mass of hemoglobin in an erythrocyte. A clinical blood test is considered the norm of 27-31 pg. The parameter is obtained by dividing the hemoglobin concentration by the number of red blood cells,
- RBC is the content of red blood cells in 1 µl of blood. A clinical blood test that shows the norm in the range of 3.5–5.2 × 106 / μl in women and 4.2–5.4 × 106 / μl in men. Their excess is due to the same reasons as too high a concentration of hemoglobin. Similarly, red blood cell deficiency is associated with its low concentration,
- Ht - otherwise HCT, or hematocrit. According to the results of a clinical analysis of the decoding blood in adults, it is determined what percentage of the volume of a given body fluid is erythrocytes. For men, the norm is 40-54%, and for women 37-47%. Sources of too high or too low ratio are exactly the same as in the case of RBC or HBL,
- MCHC - determines the average concentration of hemoglobin in an erythrocyte.
Acceptable values are 32 to 36 g/dL. Higher levels are very rare, most often in the course of a disease called hereditary spherocytosis. If the blood count is too low, it could mean thalassemia or iron deficiency,
- RC - that is, the number of reticulocytes, which are the immature form of red blood cells. They should be approximately 0.5–1.5% of the number of mature blood cells, that is, approximately 5–15 ppm. In peripheral blood, this indicator reflects the productivity of the bone marrow. Therefore, an increase in this indicator is most often associated with earlier blood loss, hemolytic anemia, vitamin B12 intake or iron deficiency or being on a significant growth, as well as splenectomy. Its decline, in turn, may be due to untreated vitamin B12 deficiency. nine0560
- RDW - this abbreviation in morphology is used to determine the coefficient of variability in the volumetric distribution of erythrocytes. The norm is 11.5-14.5%. It shows the difference between single erythrocyte volume and MCV.
A wider distribution can be caused by vitamin B12, folic acid or iron deficiency, and sometimes also after transfusions.
Leukocytes in a blood test
The results of a complete clinical blood test will also help determine the parameters associated with leukocytes. A blood test will show, first of all, the general norm of leukocytes, that is, the total number of leukocytes in the blood. Their standard ranges from 4000-10000/µl. Infection is the most common cause of an increase in this number, and if the difference is large, then also cancer, such as leukemia. nine0003
However, such serious diseases are indicated by a count of at least 30,000 blood cells per µl. Sometimes an increase in this parameter is simply the result of not following the recommendations before the blood test, for example, intense exercise or eating before the collection.
A low blood count is usually the result of an acute viral infection or the onset of one. Less common causes of excess white blood cells include treatment with immunosuppressive drugs, including glucocorticosteroids or neuroleptics, severe malnutrition, or various types of leukemia. nine0003
Clinical blood test what is included - additional indicators
In addition to the total number of leukocytes, blood indicators also determine their percentage composition, that is, parameters related to their subpopulations. These include:
- Monocytes. Their number should remain at the level of 4-8% of the total number of leukocytes. An increased number of these blood cells is caused by tuberculosis, brucellosis, infectious mononucleosis, endocarditis, syphilis, typhoid fever, protozoal infections, Crohn's disease, surgical trauma, or collagenosis, although it can sometimes be a precursor to monocytic leukemia. Too few monocytes usually means an infection or is caused by certain medications. nine0560
- Basophils. Their number in leukocytes should be about 0-1%. Increased symptoms are most often a sign of serious problems with the digestive system, including chronic inflammation or ulcerative colitis, hypothyroidism or allergic diseases, and sometimes even chronic myeloid leukemia or recovery from it,
- Eosinophils - should make up about 2-4% of white blood cells.
Too high a value can be a symptom of parasitic diseases, psoriasis, allergic diseases such as bronchial asthma or hay fever, or blood diseases and others. Sometimes this is due to certain medications, such as penicillin. Too low levels of these cells can mean sepsis, dysentery, infection, or typhoid fever. It can also be the result of injury or burns. Sometimes it is also the result of an overproduction of adrenal hormones or too intense exercise. nine0560
- Lymphocytes - should make up 25-45% of the total number of leukocytes. If they are exceeded in the blood test, this may be a symptom of hyperthyroidism, as well as lymphomas, multiple myeloma or chronic lymphocytic leukemia, but in children it may be a sign of infectious diseases. Too little of them in adults is often a symptom of AIDS and sometimes other viral infections. In children, this problem may be congenital and require urgent treatment.
- Neutrophils 60-70% - they can be divided into rod-shaped, which should be 3-5%, and segmented, the norm of which is 57-65%.
Too high a number is often a symptom of a general or local infection, metabolic disorders, and blood disorders such as myeloid leukemia. nine0560
Sometimes this condition occurs after injuries, heart attacks or hemorrhages, and also in smokers and women in the third trimester of pregnancy. A low value for this parameter in a blood test usually means a viral disease such as influenza or rubella, a bacterial disease such as typhoid fever, tuberculosis or brucellosis, or a protozoan such as malaria. Sometimes this happens with bone marrow damage, acute leukemia, or when taking cytostatics. A clinical blood test is deciphered for women and men by a specialist doctor. nine0003
Platelets in a blood test
Platelets are its smallest morphotic elements. The results of a blood test will allow you to find out about such parameters as:
- PLT - that is, the total number of platelets. The norms of this parameter are 150,000-400,000 µl. Their increased number can be observed with iron deficiency, essential thrombocythemia, chronic infections, or after removal of the spleen.
This blood test also happens after exercise or during pregnancy. Too low platelet levels can, in turn, be associated with reduced production in the bone marrow, for example, in acute leukemia and due to cancer metastases in the bone marrow, in autoimmune diseases, when taking painkillers and antibiotics, as well as the destruction of platelets by toxins. nine0560
- MPV - means the average volume of platelets and normally it is 7.5-10.5 fl. If this parameter is too low, this may indicate a decrease in platelet production,
- PDW - this abbreviation in the results of a blood test indicates the variability in the volume of her platelets. However, there is no greater diagnostic value.
It is worth remembering that the doctor must interpret the transcript of the clinical blood test in adults according to the table. The above information cannot replace a visit to a specialist. nine0003
Blood test - how to prepare
In order for the results of a blood test to be reliable, it is necessary to properly prepare the patient for the test.
